ABSTRACT
OBJECTIVE@#To investigate the expression of X-chromosome-linked inhibitors of apoptosis (XIAP) XIAP in laryngeal squamous carcinomas and the relationship between the expression of XIAP and clinical biological behaviors.@*METHOD@#Paraffin-embedded tissue specimens used for this study were obtained from 50 patients with laryngeal squamous carcinomas. The patients had received neither chemotherapy nor radiation therapy before tumor resection. Using immunohistochemical staining for the paraffin sections (SP methods), we examine the expression of XIAP protein in laryngeal squamous carcinomas and normal laryngeal tissues, investigate the connection of the XIAP expression with the clinicopathological parameters.@*RESULT@#The expression of XIAP protein was observed mainly in the cytoplasm and nucleus. The staining color was dark brown. The expression of XIAP is remarkably higher in laryngeal squamous carcinomas than that in normal laryngeal tissue specimens. The statistical analysis revealed that in laryngeal squamous carcinomas XIAP expression had no relationship with the elements such as age, sex, smoking history, tumor site and lymph node metastases. However, there is significant correlation between XIAP expression and tumor clinical stage, T stage and pathological stage (P < 0.05).@*CONCLUSION@#XIAP is expressed higher in laryngeal squamous carcinomas than in normal laryngeal tissues. The level of XIAP expression is associated with tumor clinicopathological characteristics in laryngeal squamous carcinomas. While tumor growth and malignancy increased, the expression of XIAP was up-regulated in laryngeal squamous carcinomas. It may play a role of anti-apoptosis in the process of carcinogenesis and development in laryngeal squamous carcinomas.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , Laryngeal Neoplasms , Metabolism , Pathology , Neoplasm Staging , X-Linked Inhibitor of Apoptosis Protein , MetabolismABSTRACT
OBJECTIVE To explore the role of Survivin on radiotherapy inducing apoptosis and the effect of Survivin antisense oligodeoxynucleotides(ASODN)on the growth,apoptosis and radiotherapy sensitivity in Hep-2 cells.METHODS Survivin ASODN was synthesized and thansfected into hep-2 cells with lipofectin.The experiment consisted of three steps.①The efficiency of transfection was measured with flow cytometry technique.②The expression of survivin mRNA was observed with RT-PCR.③ Apoptosis and protein expression after radiotherapy was analyzed with flow cytometry.RESULTS ①Six hours after transfection of Survivin ASODN tagged with FITC into the cells,the efficiency of transfection was found to be 94% to 98% with flow cytometry.② Twenty four hours after transfection,Survivin mRNA was found decreased with the increasing of apoptosis,which presented a dose-dependent manner.③The apoptosis in the radiotherapy combined with Survivin ASODN transfected group was significantly higher than that in radiotherapy group only(P